If there is negative pressure, proceed with the injection. The aim of this technique is to administer material into the space surrounding the abdominal organs, avoiding injection directly into any organ. Retinal degeneration induced in adult mice by a single intraperitoneal injection of nethylnnitrosourea katsuhiko yoshizawa 1,maki kurokuwata 1,2,tomo sasaki 1,3,yenchang clark lai 1,sayaka kanematsu 1, hisanori miki 1,ayako kimurakawanaka 1,norihisa uehara 1,takashi yuri 1, and airo tsubura 1. Evaluating stress and accuracy of different intraperitoneal injection. In order to optimize the administration of lactate for studies in mouse models, we compared blood lactate dynamics after intraperitoneal ip and subcutaneous sc injections. Intraperitoneal injection in the mouse although widely used as a means of administering substances, particularly injectable anaesthetics, this is an inherently unreliable technique, since inadvertent injection of some material into the gut, abdominal fat and subcutaneous tissues is a relatively frequent occurrence. We found a 12% error in the placement of intraperitoneal injections of mice with the oneman procedure ofinjection. Intraperitoneal injection of lipopolysaccharide induces. Pdf evaluation of a novel technique for intraperitoneal injections. Intraperitoneal injection of pristane 2,6,10,14 tetramethylpentadecane is a standard technique for obtaining monoclonal antibodyenriched ascitic fluid. Restrain mice with their abdomen exposed uppermost and their head pointed. For example, rabbits, cats, and dog can receive 10 20 mlkg lrs or 0. Be able to understand and recognize aspiration material. Get a printable copy pdf file of the complete article 285k, or click on a page image below to browse page by page.
Data available for intraperitoneal injection of 70% or 100% ethanol in mice suggests 4560s 75th percentile to achieve lorr and 3. The intramuscular administration is not recommended. Subcutaneous sc or sq injection in rats and mice sop. However, if proper placement of the needle occurs, this is not necessary. Errors ranging from 21 to 28% were obtained, independent ofthree investigators at two different times, all using.
Intraperitoneal injection definition of intraperitoneal. Intraperitoneal administration of a novel tatbdnf peptide. Recommended methods of anesthesia, analgesia, and euthanasia for laboratory animal species note. Unlimited viewing of the articlechapter pdf and any associated supplements and figures. The following list has been compiled from a variety of sources. An intraperitoneal injection, therefore, is an injection that introduces liquid into the cavity surrounded by the membrane. Appswe and wild type appwt mice at 12 months of age were given a daily intraperitoneal injection with the tatbdnf peptide pep, 2. Be able to consistently perform successful intraperitoneal injections on 10 mice on two consecutive occasions, performing autopsies to. Full text full text is available as a scanned copy of the original print version. Half of the volume of your dluciferin substrate can be injected on one side of the animal and the other half of the volume on the opposite side. Please see the references listed at the bottom of the page for more information. Pdf intraperitoneal injection of tetracyclines protects. Proper method for intraperitoneal injections perkinelmer.
Prepare the syringe by placing the needle on the syringe. Intraperitoneal injection of glucose if a lamb greater than 5 hours old is suffering from hypothermia, it is essential to provide it with an energy source prior to warming. Mice were sacrificed within 15 to 30 minpostinjection. Those trained persons listed on an approved animal care committee protocol performing the procedure. Pdf evaluation of a novel technique for intraperitoneal.
Lactate treatment has shown a therapeutic potential for several neurological diseases, including alzheimers disease. Fviii null or vwf null mice were infused with plasma. Administration by injection research at penn state. This document has been designed by the ulam veterinary staff as a guideline for sedation, anesthesia, and analgesia of laboratory mice. For intraperitoneal injections in fish, the animals are restrained on their side on a. Intraperitoneal ip injection in rats and mice sop page 5 of 6 for mice. Evaluation of a novel technique for intraperitoneal injections in mice article pdf available in lab animal 4411.
Listed below are recommended injection volumes for specific injection routes. Intraperitoneal immune cell responses to eubacterium. For shortterm and immediateeffect studies or when conversion of tamoxifen by the liver is to be avoided, 4oht can be used directly. Deviations from recommended injection volumes and routes require prior iacuc approval. Guidelines on anesthesia and analgesia in mice research. This will help prevent hypoglycemic seizures that can occur when a lamb is warmed up. Results using 165 mice, 24%received the ip injection in a place other than the peritoneal cavity.
Pull back on the plunger to ensure negative pressure prior to injecting. Retinal degeneration induced in adult mice by a single. The highest activity uptake was measured in the liver and spleen, followed by the femur, aorta, and in the peritoneal macrophages in the case of intraperitoneal injection. As expected, the intraperitoneal drug concentration was much higher approximately 100. It is more often applied to animals than to humans. Good practice administration routes and volumes for mice. This is not intended to be an inclusive tutorial on all possible drug combinations that can be used in mice. Jan 19, 2012 intraperitoneal injection in mice duration. Intraperitoneal injection is a common route for parenteral administration of drugs in rodents. Guidelines on anesthesia and analgesia in mice research a to z. Introduction the intraperitoneal injection the intraperitoneal i. Intraperitoneal ip injection in rats and mice sop ubc animal. Although this technique is most commonly used to inject animals like laboratory mice with medications, certain groups of people can also receive this form of injection.
Please refer to the guidelines for injections in rodents and rabbits, virginia tech office of the university veterinarian for recommended volumes and needles sizes. Intraperitoneal injection article about intraperitoneal. Evaluating stress and accuracy of different intraperitoneal injection techniques in mice research project university of utrecht. Some sites, such as footpad injection of freunds complete adjuvant, intrasplenic injection and.
A serious consequence associated with this technique, however, is the puncture of vital organs such. Intraperitoneal immune cell responses to eubacterium saphenum in mice mohammad abdus salam, michiko sato, and etsuro hoshino department ofomi microbiology, niigata university school ofdentistry, niigata. However, pristane also induces plasmacytomas and an erosive arthritis resembling rheumatoid arthritis in balbc mice, probably as a consequence of enhanced interleukin 6 production. Safety studies of the intraperitoneal injection of e1a. Urethane administered to mice by intraperitoneal injection caused tumors of the thymus thymoma in both sexes and the blood vessels in females, in addition to liver and lung tumors, as observed in earlier studies dahl et al. Administration of substances to laboratory animals.
Draw up, into the syringe and needle, the amount of solution to be administered. Evaluation of a novel technique for intraperitoneal injections in mice. Intraperitoneal ip injection in rats and mice sop page 4 of 6 mouse rat 6. Intraperitoneal injection an overview sciencedirect topics. Intraperitoneal immune cell responses to eubacterium saphenum. Intraperitoneal injection of mice applied and environmental. Recommended needle sizes and injection sites for various species are indicated. The laboratory mouse handbook of experimental animals. Recommended needle sizes and injection sites for various species are indicated in table 2. Pharmacokinetic advantage of intraperitoneal injection of. Primary intravenous iv injection site for the mouse is the right and left lateral tail vein. Considering the risks and safety of intraperitoneal injections.
Intraperitoneal injection the administration of material into the peritoneal cavity is frequently performed in mice. Miner, jacqueline koehler, and laura greenaway department of infectious diseases, human research and development laboratories, dow chemical company, indianapolis, indiana 46077. Cancer research 47, 26622667, may 15, 1987 intraperitoneal xenografts of human epithelial ovarian cancer in nude mice bruce g. In conclusion, intraperitoneal injection of omt could beneficially decrease the cciinduced mechanical allodynia and thermal hyperalgesia, antagonize the effect of nmda, synergistic enhance the effect of mk801 and infenprodil, led to a marked decrease in nmda nr2b, perk and pcreb induced by cci in the spinal cord in mice. The final biodistribution pattern of nphdl 59fespio after intravenous and intraperitoneal application in mice looked similar. This standard operating procedure sop describes the procedure of intraperitoneal ip injection in rats and mice.
Intraperitoneal injection of tetracyclines protects mice from lethal endotoxemia downregulating inducible nitric oxide synthase in various organs and cytokine and nitrate secretion in blood. Integrated systems and technologies chemotherapyinduced macrophage in. Tilt the mouse with its head slightly toward the ground so that its head is lower than its hind end. Avidin targeting of intraperitoneal tumor xenografts jnci. In this study, intraperitoneal ip injection was used to mimic the human administration route. The intramuscular administration is not recommended, as the muscle of the mouse is too small. The laboratory mouse handbook of experimental animals usp. The ld 50 of thymoquinone in rats was determined to be 57. Mice, injected with a single dose of tetracycline base tetb. In general, it is preferred when large amounts of blood replacement fluids are needed or when low blood pressure or other problems prevent the use of a suitable blood vessel for intravenous injection. Induction of lupusassociated autoantibodies in balbc mice. Plasma collected at 1 h after the challenge with sswp was used in the mmcp1 protein analysis using an elisa method described previously 42. Auranofinbased analogues are effective against clear cell. Without habituation, rats and mice have increased blood pressure and heart.
A video demonstration of the retroorbital injection route is available on the arp website. We have tested whether tetracyclines tets are able to protect mice from lipopolysaccharide lpsinduced shock, a cytokinemediated inflammatory reaction. Results of intraperitoneal and oral administration of thymoquinone in mice are given in tables1a and 1b, respectively. The most used method is intraperitoneal injection, because the amount of administered compound can be better controlled, but delivery by oral gavage is also possible. Recommended methods of anesthesia, analgesia, and euthanasia. Our initial studies on the ip injection of mice, using 26 gauge, 0. Intraperitoneal ip injections should be made into the animals right abdominal quadrant so as to avoid penetrating the cecum on the left side. Procedure summary and goal describes procedure for the administration of fluids or compounds into the abdominal cavity.
In a previous study, 14 mice were exposed to swcnts through intravenous iv injection to determine whether there were any adverse effects caused by swcnts on the mouse brain and also to find a safe application dosage if possible. This is consistent with the trend of greater effect on the nervous system of. Oct 14, 2015 appswe and wild type appwt mice at 12 months of age were given a daily intraperitoneal injection with the tatbdnf peptide pep, 2. The her2neu also called cerbb2 protooncogene is overexpressed in many human cancer cells, including those of breast cancer and ovarian cancer. Intraperitoneal injection in mice research at penn state. We explored whether infusion of vwf and fviii by either intraperitoneal i. Pdf series of pictures showing 1 using a wire cage lid to aid in mouse. Intramuscular injection in deltoid muscle with ztrack. Asm journals are the most prominent publications in the field, delivering uptodate and authoritative coverage of both basic and clinical microbiology. Some sites, such as footpad injection of freunds complete adjuvant, intrasplenic injection and intra lymph node injection. Intraperitoneal injection of lipopolysaccharide lps. Method of intraperitoneal injection in wistar rat duration. In contrast, the time to lorr is considerably longer with xylazineketamine, taking 6. Intraperitoneal injection in the mouse procedures with care.
Good practice administration routes and volumes for mice, rats, and rabbits are indicated in table 1. Four tumor models one ovarian, one lung, and two colon were established in nude mice by intraperitoneal injection of cultured cancer cells. Be able to locate the correct injection site and understand the reason why this site is preferred 4. Intraperitoneal injection or ip injection is the injection of a substance into the peritoneum body cavity. This allows the abdominal viscera to shift cranially and minimize accidental puncture of abdominal organs at site of injection. Subcutaneous sc or sq injection in rats and mice sop page 2 of 2 procedure 1. In this study, we tested the ability of avidin, labeled through conjugation to radioactive biotin a b vitamin, to target intraperitoneal tumors.
Intraperitoneal injection is not a suitable administration. All animal users performing ip injections in rodents must. The table includes medications and dosages currently recommended for each species. Blood lactate dynamics in awake and anaesthetized mice after. Dec 01, 1994 intraperitoneal injection of pristane 2,6,10,14 tetramethylpentadecane is a standard technique for obtaining monoclonal antibodyenriched ascitic fluid. A group of control mice received alum only for the injection. They can be used as referenced descriptions for procedures on iacuc protocols. Intraperitoneal injection in the mouse although widely used as a means of administering substances, particularly injectable anaesthetics, this is an inherently unreliable technique, since inadvertent injection of some material into the gut, abdominal fat and subcutaneous tissues is a. Intraperitoneal xenografts of human epithelial ovarian cancer. Our initial studies on the ip injection of mice, using 26gauge, 0.
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